The human cancer cell line, SKMES1 and A549 were obtained from the ATCC/LGC standard (Teddington, Middlesex, England, UK). Recombinant human hepatocyte growth factor was a gift from Prof. Toshikazu Nakamura (Osaka University, Japan). The recombinant EGF comes from Sigma-Aldrich (Poole, Dorset, England, UK). Small inhibitors of the HGF receptor SU11274 (IC50 of cMET at 20 nM) and PHA6665752 (IC509 nM of cMET), And AG490, a small inhibitor of EGFR kinase (IC50 2µM of EGFR), from Tocris Chemicals (Bristol, England, UK). Antibodies against human HGF, human cMET (SC-10), and phospho-CMET (SC-34088) were obtained from Santa Cruz Biotechnologies Inc. (Santa Cruz, CA, USA). A fluorescence-labeled secondary antibody conjugated by horseradish peroxidase (HRP) was obtained from SigmaAldrich (Poole, Dorset, England, UK).

The traditional Chinese medicine formula Yangzheng Xiaoji was obtained from Yiling Pharmaceutical (Shijiazhuang, Hebei, China). The formula contains the following 16 ingredients: Ginseng C.A.M. Astragalus and privet. Turmeric, Ganoderma lucidum, Gynostemma gynostemma, white art, Lelabra, white flower snake tongue, Tuckahoe, digitalis. , Artemisia scoparia, Bge.) Ki, Cynanchum paniculatum Kitag, Eupolypaga sinensis Walker, and domestic chicken (Gallus domesticus Brisson). Found a high degree of consistency in chemical fingerprints from batch to batch. Attached file 1 shows fingerprints taken from 10 batches of positive notes over a period of 2 years (attached file 1).

YangZheng XiaoJi extract, called DME25, was prepared from YangZhengXiaoji using a DMSO-based method as previously reported [8,10,34]. Simply, the powder prepared by YangZheng XiaoJi is added to a pure DMSO solution (Sigma-Aldrich). It is placed on a wheel (Labinco BV, Wolf Laboratory, York, England, UK) in a cold room at 4°C and 100 rpm for 12 hours [8,10]. The preparation was centrifuged at 15000×g at 4°C for 20 minutes. The supernatant was carefully removed and filtered using a disc filter (pore size 0.20μm, Sartorius Stedim, Sartorus, Epson, Surrey, England, UK). The extract was diluted in a balanced salt solution (BSS) and standardized by quantifying the optical density of the preparation at a wavelength of 450nm using a spectrophotometer (Biotek, Wolf Laboratory). A masterbatch preparation producing 0.25OD at 450nm will be stored as a masterbatch and named DME25 for subsequent experiments. DME25 dilution less than 1:40 showed no effect on the growth of lung cancer cells. In this study, we used a dilution range between 1:100 and 1:2000.